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hepg2 cells  (CLS Cell Lines Service GmbH)


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    Structured Review

    CLS Cell Lines Service GmbH hepg2 cells
    Hepg2 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 95/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hepg2+cells/HepG2+Cells/custom%40300198%4042692359
    Average 95 stars, based on 152 article reviews
    hepg2 cells - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Reporter Assay:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we

    Transfection:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we

    Cell Culture:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we

    Luciferase:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we

    Thin Layer Chromatography:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we

    Modification:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we

    Variant Assay:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we

    Phospho-proteomics:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we

    Gene Expression:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we

    Targeted Gene Expression:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we

    Expressing:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we

    Mutagenesis:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we

    Stable Transfection:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we

    In Vitro:

    Article Title: IL-13 ameliorates hepatocellular steatosis via IL-13Rα1/JNK/FOSL2 signaling pathway.
    Article Snippet: l acetic acid 22:3 (V/V), blue nozzle, spraying level 3; TLC Plate Heater, 105 °C, 5 min) to 545 distinguish from coeluted phosphatidylinositol. 546 547 Dual-Luciferase Reporter Assay 548 HepG2 cells (CLS #330198; ordered 2019; cryostocks ≤10 passages) were cultured in DMEM with 10% 549 FCS and 1% penicillin/streptomycin at 37°C and 5% CO₂ (Anprotec, Bruckberg, Germany). Cells (4 × 550 10⁴/well) we



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    Image Search Results


    The effects of SERBP1 depletion on PPARγ target genes in HepG2 cells HepG2 cells were treated with scrambled short hairpin RNA (sh Scr) or shRNA against SERBP1 (sh SERBP ). The cells were incubated with or without 0.5 mM oleic acid (OA). Total RNA was isolated, and RT-PCR was performed to determine the mRNA levels of SERBP1 (A), PLIN2 (B), PLIN3 (C), and CD36 (D), normalized to GAPDH . The expression level in OA-treated sh Scr was set to 1.0. The results are shown as scattered dot plots (mean ± SD). ∗∗ p < 0.01, one-way ANOVA followed by Tukey-Kramer post hoc test.

    Journal: iScience

    Article Title: NEK2A regulates PLIN2 expression through a SERBP1 dependent pathway

    doi: 10.1016/j.isci.2026.116254

    Figure Lengend Snippet: The effects of SERBP1 depletion on PPARγ target genes in HepG2 cells HepG2 cells were treated with scrambled short hairpin RNA (sh Scr) or shRNA against SERBP1 (sh SERBP ). The cells were incubated with or without 0.5 mM oleic acid (OA). Total RNA was isolated, and RT-PCR was performed to determine the mRNA levels of SERBP1 (A), PLIN2 (B), PLIN3 (C), and CD36 (D), normalized to GAPDH . The expression level in OA-treated sh Scr was set to 1.0. The results are shown as scattered dot plots (mean ± SD). ∗∗ p < 0.01, one-way ANOVA followed by Tukey-Kramer post hoc test.

    Article Snippet: HuH7 cells were obtained from JCRB Cell Bank, and HepG2 cells were obtained from ATCC.

    Techniques: shRNA, Incubation, Isolation, Reverse Transcription Polymerase Chain Reaction, Expressing